• Postdoctoral Position in Proteomics

    Posted Date 2 months ago(6/14/2019 6:51 AM)
    Job ID
    2019-2051
    # Positions
    1
    Type
    Full-Time 40
    Location
    US-SC-Charleston
    Security Clearance Level
    N/A
  • Responsibilities

    CSS is seeking to fill a one year postdoctoral position to work on our contract with NOAA’s National Centers for Coastal Ocean Science (NCCOS) in collaboration with the National Institute of Standards and Technology at the Hollings Marine Laboratory in Charleston, SC. The project will develop and employ cutting-edge mass spectrometry-based proteomic techniques to investigate the causes of stony coral tissue loss disease (SCTLD) and identify targets of mitigation and treatment. To date, the SCTLD pandemic has infected roughly half the corals in waters off Florida. It is an infectious water-borne disease of unknown etiology with high-mortality that is quickly spreading across coral stocks. The study will include analyzing samples from diseased and healthy corals as well as the microbiome composition of the coral holobiont and surrounding environment. Utilizing state-of-the-art instrumentation at NIST Charleston (including high-resolution mass spectrometry), proteomics will be used to evaluate the molecular progression of SCTLD in different coral species and identify candidate markers of disease, while metaproteomics will be used to provide insight into changes in the microbial community composition and function with disease progression. The post-doctoral candidate should be well-versed in running LC-MS/MS and downstream metaproteomic bioinformatics pipelines. Additional expertise in metagenomics, metatranscriptomics and microbiology is beneficial but not required. We expect these results to directly inform mitigation and policy decisions related to the SCTLD outbreak, in addition to drive new hypotheses and research directions. Analytical instrumentation includes Thermo Scientific Fusion Lumos orbital trap system and a Q-Exactive orbital trap.

     

    The research is primarily laboratory based but does have a portion of work that involves field missions to investigate coral and environmental conditions at specific locations experiencing SCTLD, domestically and internationally. The incumbent is expected to participate both technically and intellectually in the preparation of written reports of research findings and presentations when appropriate. The research will be conducted as a member of an interdisciplinary research team of scientists under the direction of the principal investigator.

     

    DUTIES AND RESPONSIBLITIES

     

    1. Apply scientific knowledge, techniques, instrumentation and methods necessary to achieve project goals, including designing and conducting experiments, and analyzing and interpreting data under the general oversight of the principal investigator.
    2. Design and conduct near-term experiments on SCTLC, and these can include transcriptomics and proteomics of SCTLD affected species and trials of novel intervention strategies.
    3. Participate as needed on weekend duty to conduct extended duration experiments. Emergency situations with storms and/or power outages also may require the incumbent to assist others if coral facility staff are not available or additional help is needed.
    4. Keep accurate written records of all methods, procedures, and experimental results, documents observations of unusual or abnormal appearance, and prepare data reports. Laboratory data acquisition also often involves photodocumentation and photomicrograph documentation.
       

    Qualifications

    MINIMUM REQUIREMENTS: 

    1. PhD degree in one of the biological sciences with experience related to biochemistry, proteomics in disease diagnosis and treatment
    2. Demonstrated laboratory experience in at least three of the following areas: 
      • biochemistry - protein chemistry
      • proteomics
      • invertebrate pathology
      • ‘omics data analysis (i.e., genomic, proteomic, metabolomics)
      • bioinformatics
    3. Significant experience analyzing and drawing inferences from experimental data,
    4. Demonstrated ability to communicate effectively both orally and in writing, and documenting findings in the form of records, reports, and journal manuscripts.
    5. Experience in statistics in genomics, transcriptomics or proteomics is preferred.
    6. Demonstrated ability to run LC-MS/MS and downstream metaproteomic bionformatics piplines. 
       

     

    CSS, Inc. is an Equal Opportunity Employer, all qualified applicants will receive consideration for employment without regard to race, color, religion, gender, gender identity or expression, sexual orientation, national origin, genetics, disability, age, or veteran status.

     

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